Characterization of genetic determinants for growth and structure of Listeria monocytogenes biofilms / by Nicholas Choy.
Choy, Nicholas Chiau-Yoong
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"This thesis investigated wild-type 'Listeria monocytogenes ' EGD and five of its corresponding mutant strains (M1, M4, M7, M11 and M14) for their ability to form biofilms on glass at 22C and 37C, with and without shear force. Cell counts of 24 h-old biofilms ranged from 105 to 107cfu/cm2. For all strains investigated, biofilms grown without shear force at 22C had higher mean biofilm cell counts than at 37C (P<0.05). Biofilms of wild-type ' L. monocytogenes' EGD and its mutant M11 ('clpB' gene deleted) were further analyzed with scanning electron microscopy (SEM). SEM imaging of biofilms grown at 37C without shear force showed that the wild-type strain EGD produced significantly more biofilm cells than its mutant M11.