Please use this identifier to cite or link to this item: https://knowledgecommons.lakeheadu.ca/handle/2453/1757
Title: The relationship between whole blood, plasma and total blood lactate at various exercise intensities
Authors: Newhouse, Donna
Keywords: Glycolysis;Factors affecting blood lactate;Acid-base balance;Plasma volume;Whole blood and plasma lactate discrepancies;Mechanisms for cell to plasma lactate gradients
Issue Date: 1992
Abstract: The purpose of this study was to examine the relationship between lactate concentrations derived from three sources: whole blood (WBLa), plasma (Pla) and total blood (TBLa) during an incremental bicycle test to exhaustion. Regression equations were computed enabling the prediction of one source from another. Thirty-two male subjects (16-25 yrs) participated in this study. Subjects performed a graded bicycle ergometer exercise test to exhaustion. Consecutive blood samples were drawn via an indwelling venous catheter at two minute intervals throughout the test, immediately post, and 5 minutes post exercise. Blood was analyzed for: WBLa, Pla, TBLa (YSI Lactate Analyser Model 23L), hemoglobin, hematocrit, bicarbonate and pH. Correlations were calculated for all variables. Simple and multiple regression equations were computed across exercise intensities. A one-way ANOVA was performed to determine if any significant differences existed between these three indices. This study illustrated that WBLa, Pla and TBLa concentrations, differed significantly and as exercise intensity increased so did the discrepancy. The regression equations were PLa=1.568(WBLa) + 0.149; WBLa=0.887(TBLa) + 0.015; PLa=1.208(TBLa) + 0.093. Although highly correlated to lactate values, the predictor variables: hemoglobin, hematocrit, bicarbonate and pH did not enhance the prediction equation.
URI: http://knowledgecommons.lakeheadu.ca/handle/2453/1757
metadata.etd.degree.discipline: Kinesiology
metadata.etd.degree.name: Master of Science
metadata.etd.degree.level: Master
metadata.dc.contributor.advisor: Thayer, Robert
Appears in Collections:Retrospective theses

Files in This Item:
File Description SizeFormat 
NewhouseD1992m-1b.pdf3.1 MBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.