Please use this identifier to cite or link to this item:
https://knowledgecommons.lakeheadu.ca/handle/2453/4194
Title: | A multiomic approach to paleogenetic investigation of ancient North American bison |
Authors: | Boyle, Joseph |
Keywords: | Structure and nature of DNA;Ancient DNA;Structure of protein;Proteomics;Multiomics;Bison in North America |
Issue Date: | 2018 |
Abstract: | Twelve ancient bison bone samples from north-central North America were examined using genetic and proteomic sequencing to determine relationships to other bison populations. Mitochondrial DNA sequences suggest a genetic affinity that most closely matches populations from contemporaneous bison populations located in central North America. Proteomic sequencing by liquid chromatography tandem mass spectrometry could only resolve relationships to broad taxa and could not determine intra-specific relationships. Also, a novel multiple and simultaneous extraction protocol is presented to extract material suitable for both genetic and proteomic analysis from the same bone sample. It was also found that human serum albumin can be used as a replacement for bovine serum albumin as an effective additive to improve DNA amplification. In addition, chloroform alone can be used as an efficient organic solvent for the purification and separation of protein. |
URI: | http://knowledgecommons.lakeheadu.ca/handle/2453/4194 |
metadata.etd.degree.discipline: | Biology |
metadata.etd.degree.name: | Master of Science |
metadata.etd.degree.level: | Master |
metadata.dc.contributor.advisor: | Matheson, Carney |
metadata.dc.contributor.committeemember: | Law, David Hamilton, Scott |
Appears in Collections: | Electronic Theses and Dissertations from 2009 |
Files in This Item:
File | Description | Size | Format | |
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BoyleJ2018m-1b.pdf | 4.59 MB | Adobe PDF | ![]() View/Open |
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