Lakehead University Library Logo
    • Login
    View Item 
    •   Knowledge Commons
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations from 2009
    • View Item
    •   Knowledge Commons
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations from 2009
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    quick search

    Browse

    All of Knowledge CommonsCommunities & CollectionsBy Issue DateAuthorTitleSubjectDisciplineAdvisorCommittee MemberThis CollectionBy Issue DateAuthorTitleSubjectDisciplineAdvisorCommittee Member

    My Account

    Login

    Statistics

    View Usage Statistics

    A multiomic approach to paleogenetic investigation of ancient North American bison

    Thumbnail

    View/Open

    BoyleJ2018m-1b.pdf (4.482Mb)

    Date

    2018

    Author

    Boyle, Joseph

    Degree

    Master of Science

    Discipline

    Biology

    Subject

    Structure and nature of DNA
    Ancient DNA
    Structure of protein
    Proteomics
    Multiomics
    Bison in North America

    Metadata

    Show full item record

    Abstract

    Twelve ancient bison bone samples from north-central North America were examined using genetic and proteomic sequencing to determine relationships to other bison populations. Mitochondrial DNA sequences suggest a genetic affinity that most closely matches populations from contemporaneous bison populations located in central North America. Proteomic sequencing by liquid chromatography tandem mass spectrometry could only resolve relationships to broad taxa and could not determine intra-specific relationships. Also, a novel multiple and simultaneous extraction protocol is presented to extract material suitable for both genetic and proteomic analysis from the same bone sample. It was also found that human serum albumin can be used as a replacement for bovine serum albumin as an effective additive to improve DNA amplification. In addition, chloroform alone can be used as an efficient organic solvent for the purification and separation of protein.

    URI

    http://knowledgecommons.lakeheadu.ca/handle/2453/4194

    Collections

    • Electronic Theses and Dissertations from 2009

    Lakehead University Library
    Contact Us | Send Feedback

     


    Lakehead University Library
    Contact Us | Send Feedback