Lakehead University Library Logo
    • Login
    View Item 
    •   Knowledge Commons
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations from 2009
    • View Item
    •   Knowledge Commons
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations from 2009
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    quick search

    Browse

    All of Knowledge CommonsCommunities & CollectionsBy Issue DateAuthorTitleSubjectDisciplineAdvisorCommittee MemberThis CollectionBy Issue DateAuthorTitleSubjectDisciplineAdvisorCommittee Member

    My Account

    Login

    Statistics

    View Usage Statistics

    Cystathionine gamma-lyase/hydrogen sulfide system and glucose homeostasis

    Thumbnail

    View/Open

    ZhangL2012d-1a.pdf (4.456Mb)

    Date

    2013-02-12

    Author

    Zhang, Ling

    Degree

    Ph.D.

    Discipline

    Biotechnology

    Subject

    Hydrogen sulfide
    Cystathionine gamma-lyase
    Insulin secretion
    Glucose regulation
    Diabetes

    Metadata

    Show full item record

    Abstract

    Research in the last twenty years has transformed the role of hydrogen sulfide (H2S) being perceived from a toxic gas to a gaso-transmitter with diverse physiological and pathological significance. Cystathionine gamma-lyase (CSE) is the major enzyme responsible for the endogenous production of H2S in pancreatic β cells and liver cells, which are two key types of cells to regulate glucose level. The hallmarks of type 2 diabetes mellitus consist of insulin resistance, pancreatic β-cell dysfunction, and increased endogenous glucose production by liver. Pathophysiological implications of the CSE/H2S system in both type 1 and type 2 diabetes as well as diabetic complications have been reported before. Previous studies in our laboratory have shown that endogenous H2S mainly produced from CSE inhibited insulin release via activating KATP channels and decreased H2S production was observed after exposure to elevated glucose level in pancreatic β cells. However, the effect of glucose on CSE gene expression in pancreatic β cells and the underlying mechanism have not been thoroughly explored. Liver, an important organ to control glucose level, has relatively high amount of H2S production compared to other organs and CSE has been reported to be the major enzyme responsible for it. The aim of the current study was to investigate the effect of glucose on CSE expression in INS-1E cells (insulin-secreting β cells) and the role of H2S on basal and insulin-stimulated glucose uptake, glycogen synthesis and gluconeogenesis in human hepatoma HepG2 cells as well as the underlying mechanisms.

    URI

    http://knowledgecommons.lakeheadu.ca/handle/2453/443

    Collections

    • Electronic Theses and Dissertations from 2009

    Lakehead University Library
    Contact Us | Send Feedback

     


    Lakehead University Library
    Contact Us | Send Feedback