dc.description.abstract | Native freshwater microalgae play a crucial role in biofuel production due to their unique
characteristics and environmental suitability. This study investigated the diversity of
microalgae species in various freshwater ecosystems across Northwestern Ontario, Canada and
evaluated the nutrient removal, bioremediation and subsequent biomolecule production.
Microalgae are known for their biochemical diversity, synthesizing an extensive range of
compounds including proteins, lipids, carbohydrates, vitamins, pigments, and bioactive
molecules In Chapter One, we provided a comprehensive review on the microalgae cultivation
and harvesting techniques along with pretreatment methods for the extraction of biomolecules.
In Chapter Two, we isolated 75 potential microalgal isolates and characterized these stains to
identify those with high potential for lipid and biomolecule production capacity through a
combination of microscopy, molecular techniques, and biochemical analysis and identified the
optimal light condition for the biomass and lipid production. Among these isolated strains, a
five microalgae strains with higher lipid production and growth were identified using molecular
and morphological approaches. Among four different photoperiods, 16L:8D light/dark cycle
produced significantly higher biomass and lipids for Chlorella sorokiniana, Chlorella vulgaris,
and Chlamydomonas sp. [...] | en_US |