Lakehead University Library Logo
    • Login
    View Item 
    •   Knowledge Commons
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations from 2009
    • View Item
    •   Knowledge Commons
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations from 2009
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    quick search

    Browse

    All of Knowledge CommonsCommunities & CollectionsBy Issue DateAuthorTitleSubjectDisciplineAdvisorCommittee MemberThis CollectionBy Issue DateAuthorTitleSubjectDisciplineAdvisorCommittee Member

    My Account

    Login

    Statistics

    View Usage Statistics

    Recovering the proteome of archived biomedical specimens

    Thumbnail

    View/Open

    SalamonA2021m-1a.pdf (1.062Mb)

    Date

    2021

    Author

    Salamon, Ashley

    Degree

    Master of Science

    Discipline

    Biology

    Subject

    Proteomics
    Formalin fixed paraffin embedded (FFPE) tissue
    FFPE brain tissue
    Biomedicine

    Metadata

    Show full item record

    Abstract

    While several proteomic studies have undertaken the challenge of analyzing formalin fixed paraffin embedded (FFPE) tissue, few have developed optimized protocols for studying FFPE human brain tissue. This study will examine if an improved SDS free proteomic method applied to FFPE human brain tissue will produce a greater number of identified proteins then assess optimized methods to produce reliable, robust and reproducible results from this tissue. A modified guanidinium hydrochloride based extraction method was employed and the extracted proteins were analyzed using LC-MS/MS, for shotgun proteomics. The FFPE human brain tissue samples were acquired from archived specimens at the Griffith University School of Anatomy. By analyzing tissue samples from human FFPE brain, we evaluated conditions used for tissue lysis, digestion, and the effects of the fixation time and storage duration on the number of proteins obtained during shotgun proteomic analysis on FFPE tissue. Comparing the identified proteins and experimental procedures to the existing FFPE tissue studies in the literature, the limitations due to protein quality were identified, optimized processes proposed, and future studies including multiomic analysis of the human brain, were outlined.

    URI

    https://knowledgecommons.lakeheadu.ca/handle/2453/4814

    Collections

    • Electronic Theses and Dissertations from 2009

    Lakehead University Library
    Contact Us | Send Feedback

     


    Lakehead University Library
    Contact Us | Send Feedback